论文部分内容阅读
本文报道了以脱脂贻贝粉为原料,采用酶解法从其酶解液中分离,提取及纯化复合氨基酸和六种单一氨基酸的工艺。确定了贻贝粉以胃蛋白酶、枯草杆菌中性蛋白酶和胰蛋白酶联合的酶解工艺,采用超滤方法去除酶解液中未水解的蛋白质等大分子物质及进行脱色处理。将超滤液通过701#[CO=]型碱性阴离子交换树脂及[H+]型1×25强酸性阳离子交换树脂脱盐,得到的脱盐液经真空浓缩、结晶、洗涤和烘干得复合氨基酸粉末,得率为20%左右(相对子贻贝粉,w/w,下同)。将超滤液通过活性炭柱吸附苯丙氨酸和酪氨酸,再用洗脱液洗下,经浓缩、结晶、洗涤和烘干得单一氨基酸成品,L-苯丙氨酸得率为2.45%,L-酪氨酸得率为1.21%。活性炭柱的通过液经732强酸性阳离子交换树脂柱及717强碱性阴离子交换树脂柱组吸附、分离、洗脱得纯 L-精氨酸组分、纯 L-谷氨酸组分、L-亮氨酸及L-缬氨酸组分。再经赶氨、浓缩、结晶、洗涤和烘干得成品,得率分别为3.01%、1.02%、1. 46%及 1. 34%。单一纯化的氨基酸产品,经氨基酸自动分析仪定性、定量测定及红外光谱分析和通过自动指示旋光仪测定其比旋度进行鉴定,证实确为本物质。复合氨基酸产品中游离氨基酸含量为90.
In this paper, the process of separating, extracting and purifying compound amino acids and six single amino acids from defatted mussel powder by enzymatic hydrolysis was reported. Determine the mussel powder to pepsin, Bacillus subtilis neutral protease and trypsin enzymatic hydrolysis process, using ultrafiltration method to remove unhydrolyzed protein hydrolyzate and other macromolecular substances and decolorization. The ultrafiltrate was desalted by using 701 # [CO =] type basic anion exchange resin and [H +] type 1 * 25 strong acid cation exchange resin, and the obtained desalted solution was concentrated in vacuo, crystallized, washed and dried to obtain composite amino acid powder , The yield is about 20% (relative to mussel meal, w / w, the same below). The ultrafiltrate was adsorbed on phenylalanine and tyrosine via activated charcoal column and then washed with the eluate. The single amino acid product was concentrated, crystallized, washed and dried to obtain a product with an L-phenylalanine yield of 2. 45%, L-tyrosine yield was 1.21%. Activated carbon column through the liquid 732 strongly acidic cation exchange resin column and 717 strong basic anion exchange resin column adsorption, separation, pure L-arginine component pure L-glutamic acid component L- Leucine and L-valine components. Then by ammonia, concentrated, crystallization, washing and drying the finished product, the yield was 3.01%, 1.02%, 1. 46% and 1. 34%. A single purified amino acid product, amino acid automatic analyzer qualitative, quantitative and infrared spectroscopy and by automatic indicator polarimeter to determine the specific rotation to identify, confirmed indeed the material. Compound amino acid products free amino acid content of 90.