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microRNA是一类在转录后调控中发挥重要作用的非编码小RNA,其靶标参与高等植物的发育、代谢、胁迫应答等生物学过程.糜子是一类重要的禾谷类作物,但目前还未见与其相关的miRNA报道.通过基于表达序列标签和miRNA前体二级结构的同源搜索,鉴定到43个新的miRN As.这些miRN As不均匀地分布于11个miRNA家族.利用psRNAtarget对这些miRN As分析表明,它们调控68个靶标基因.11个miRN As家族中的10个参与了RNA调控,表明了它们在糜子转录后调控中的基本作用.利用Northern blot对8个家族的miRN As进行了鉴定,表明本实验的预测方法是有效的.
MicroRNAs are a class of non-coding small RNAs that play an important role in post-transcriptional regulation and whose targets are involved in the biological processes of higher plant development, metabolism, stress response, etc. Millet is an important class of cereal crops but has not yet been seen Related miRNAs were reported.43 novel miRN As were identified based on homology search of expressed sequence tags and secondary structures of miRNA precursors.The miRNAs were heterogeneously distributed in 11 miRNA families.These miRNAs As analysis indicated that they regulate 68 target genes and 10 of 11 miRN As families are involved in RNA regulation, indicating their basic role in post-transcriptional regulation of broomcorn millet.The miRNAs of 8 families were analyzed by Northern blot Identification, indicating that the experimental prediction method is effective.