利用微机械加工技术研制了具有三维结构的腔型传感器 ,双腔型工作电极与Ag AgCl参比电极集成在同一微芯片上。考察了微腔型电极的电化学特性及其对H2 O2 含量的测定。并以此为基础电极制备了半乳糖、葡萄糖双腔型生物传感器。结果表明 ,该传感器可同时测定半乳糖、葡萄糖双组分 ,线性上限分别为 4 5mmol L和 4 0mmol L。而且在测试双组分过程中 ,没有观察到明显的交叉干扰现象。 The cavity sensor with three-dimensional structure was developed by micro-machining technology. The dual-chamber working electrode and Ag AgCl reference electrode were integrated on the same microchip. The electrochemical characteristics of the microcavity electrode and its determination of H2 O2 content were investigated. And based on this electrode prepared galactose, glucose dual-chamber biosensor. The results show that the sensor can simultaneously determine galactose, glucose bicomponent, the linear upper limit were 45mmol L and 40mmol L. And in the test two-component process, no significant cross-interference observed.