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目的探讨新疆维吾尔族胃病患者感染幽门螺杆菌(Helieobacter pyroli,Hp)vacA基因亚型的分布状况及与胃病的关系。方法临床收集维吾尔族人群慢性浅表性胃炎、慢性萎缩性胃炎、胃溃疡和胃癌患者胃镜活检标本,采用Hp分离培养技术对其进行培养鉴定;采用Chelex 100提取Hp基因组,聚合酶链反应-序列特异性引物(PCR-SSP)对Hp基因组DNA进行vacA基因亚型检测,并对vacA基因亚型与特定疾病间的关系进行分析。结果胃活检标本Hp培养阳性46例,上述4种病人vacAs区s1a型阳性率分别为78.6%(11/14)、63.2%(12/19)、66.7%(4/6)和85.7%(6/7),vacAs区s2型阳性率分别为0(0/14)、5.3%(1/19)、0(0/6)和14.3%(1/7),vacA m区m1b型阳性率分别为14.3%(2/14)、10.5%(2/19)、16.7%(1/6)和0(0/7),vacA m区m2型阳性率分别为78.6%(11/14)、57.9%(11/19)、50.0%(3/6)和85.7%(6/7)。4种疾病间的vacA亚型分布差异均无统计学意义(P>0.05)。结论新疆维吾尔族人群中Hp vacA亚型以s1a/m2型为主。
Objective To investigate the distribution of vacA genotypes of helicobacter pylori (Helicobacter pyroli) in patients with stomach cancer of Uygur in Xinjiang and their relationship with gastric diseases. Methods The gastroscopic biopsies of chronic superficial gastritis, chronic atrophic gastritis, gastric ulcer and gastric cancer were collected from Uyghur people. Hp isolation and culture techniques were used to identify the samples. Chelex 100 was used to extract Hp genome and polymerase chain reaction Specific primers (PCR-SSP) were used to detect the vacA genotype of Hp genomic DNA, and the relationship between vacA gene subtypes and specific diseases was analyzed. Results The positive results of Hp culture in gastric biopsy specimens were 46 cases. The positive rates of s1a in vacAs were 78.6% (11/14), 63.2% (12/19), 66.7% (4/6) and 85.7% (6) / 7). The positive rates of s2 in vacAs were 0 (0/14), 5.3% (1/19), 0 (0/6) and 14.3% (1/7) respectively. The positive rates of m1b in vacA m were Were 14.3% (2/14), 10.5% (2/19), 16.7% (1/6) and 0 (0/7) respectively. The positive rates of m2 in vacA m were 78.6% (11/14) and 57.9 % (11/19), 50.0% (3/6) and 85.7% (6/7). There were no significant differences in the distribution of vacA subtypes between the four diseases (P> 0.05). Conclusion Hp vacA subtype in Xinjiang Uygur population is mainly s1a / m2 type.