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目的 探讨重庆人群亚甲基四氢叶酸还原酶 (Methylenetetrahdrofolatereducatase ,MTHFR)基因多态性与结直肠癌的关系 ,研究叶酸、MTHFR基因多态性以及结直肠癌发生的交互作用。方法 ①用聚合酶链反应限制性片段多态性(PCR RFLP)技术 ,检测 82例结直肠癌患者和 82例正常对照的MTHFR基因多态性。②使用放免方法检测病例及对照血浆中的叶酸含量。结果 ①病例组叶酸水平显著低于对照组。②结直肠癌患者与对照MTHFR突变基因型率分别为 5 6 1%和 5 1 3 %,无统计学差异。结论 叶酸水平与结直肠癌的发生显著相关 ,是结直肠癌发病的保护因素。在重庆人群中MTHFR基因多态性可能不是结直肠癌发生的危险因素
Objective To investigate the relationship between MTHFR gene polymorphism and colorectal cancer in Chongqing population, and to study the interactions of folic acid, MTHFR gene polymorphism and colorectal cancer. Methods 1 Polymerase chain reaction restriction fragment polymorphism (PCR-RFLP) was used to detect MTHFR gene polymorphisms in 82 patients with colorectal cancer and 82 normal controls. 2 Use radioimmunoassay to detect folic acid in the case and control plasma. Results 1 The folic acid level in the case group was significantly lower than that in the control group. 2The rate of MTHFR mutation genotype in patients with colorectal cancer was 5 1% and 513 , , respectively, with no statistical difference. Conclusion Folic acid levels are significantly associated with the occurrence of colorectal cancer and are protective factors for the onset of colorectal cancer. MTHFR gene polymorphism may not be a risk factor for colorectal cancer in Chongqing population