抗疟新药的初筛试验,一般对每种新药的一种剂量用10只感染鼠疟原虫的小白鼠,观察有无抗疟效果,需用小白鼠数量多,工作量大。优选方法与步骤:用对分法,设5只、7只、10只共三组小白鼠分别在同一条件下接种后定期检查计算各小白鼠血内疟原虫的数量,结果,经变异数分析,均无显著性差异,说明每次用5只小白鼠与用10只的效果基本一致,可节约小白鼠用量,并减少工作 The primary screening test for new anti-malarial drugs generally uses 10 mice infected with Plasmodium falciparum at a dose of each new drug to observe whether there is any anti-malarial effect. It is necessary to use a large number of mice and the workload is large. The preferred method and procedure are: using dichotomy, set 5, 7 and 10 groups of mice to be vaccinated under the same conditions and check regularly to calculate the number of malaria parasites in each mouse. The results are analyzed by variation. There was no significant difference between the two groups, indicating that the effect of using 5 mice per time was basically the same as that of using 10 mice. This saved the use of mice and reduced the work.