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为开发栎属遗传多样性检测的SSR标记,分析了蒙古栎、无梗花栎、夏栎和欧洲栓皮栎EST-SSR的特点,结果表明蒙古栎的EST中3 209.46 bp有一个SSR,无梗花栎中每6 160.36 bp有一个SSR、夏栎中每5 883.30 bp有一个SSR,欧洲栓皮栎中每6 129.12 bp有一个SSR。蒙古栎、无梗花栎、夏栎和欧洲栓皮栎EST-SSR的平均长度分别为21.65 bp、21.1 bp、20.66 bp和20.65 bp。四种栎类中不同基元的EST-SSR的分布频率具有非常一致的特征,均是二基元、三基元和六基元的SSR分布频率最高,达20%以上。而四基元和五基元的SSR在四个种类中的分布不到0.05%。二基元的SSR中大于1%的SSR均是AG、CT、TC、GA、AT、TA基元,并且在蒙古栎、无梗花栎和夏栎中AG、CT、TC基元的分布频率最高,而在欧洲栓皮栎中是TC、GA、AG的分布频率最高;三基元的SSR中,含CAA、GAA、TCT、CTT的SSR在四种栎类中都存在。六基元的SSR中大于1%在四种栎类中出现的类型均较少,为0~4种。
In order to develop SSR markers for the genetic diversity of Quercus, EST-SSR of Quercus mongolica, Quercus mongolica, Quercus variabilis and Quercus variabilis were analyzed. The results showed that there was an SSR at 3 209.46 bp in Quercus mongolica EST, There were one SSR per 6 160.36 bp in Quercus acutissima, one SSR per 5 883.30 bp in Quercus acutissima and one SSR per 6 129.12 bp in Quercus variabilis. The average lengths of EST-SSR in Quercus mongolica, Quercus mongolica, Quercus acutissima and Quercus variabilis were 21.65 bp, 21.1 bp, 20.66 bp and 20.65 bp, respectively. The distribution frequencies of EST-SSRs of different origins in four oak species were very consistent, and the SSR frequencies of the two, three and six motifs were the highest, reaching over 20%. The SSRs of the four and five motifs were distributed in less than 0.05% of the four species. The SSRs of more than 1% of the SSRs in the two motifs were AG, CT, TC, GA, AT and TA motifs, and the distribution frequency of AG, CT and TC motifs in Quercus mongolica, The highest frequencies were found in European Quercus variabilis, while the highest frequencies were found in European Quercus variabilis. GA, AG and AG were the most frequently distributed in the European Quercus variabilis. SSRs containing CAA, GAA, TCT and CTT in the three-member SSR were found in all the four oak species. More than 1% of the SSRs of the six-member SSRs were found in all the four oaks with fewer types of 0-4.