论文部分内容阅读
为了解黑龙江省绥化市某规模化奶牛场腹泻犊牛大肠杆菌分离株的耐药性及耐药基因携带情况,在2016年4月,收集该牛场群发腹泻犊牛的8份粪便,通过细菌分离鉴定,生化实验等鉴定方法,确定牛群腹泻的病因为大肠杆菌感染。结果显示对氟苯尼考,多粘菌素B高度敏感,对青霉素具有较强的耐药性,耐药率达100%,其四环素、环丙沙星、恩诺沙星耐药率达75%;应用PCR方法检测大肠杆菌耐药基因,共检出5种耐药基因,分别为bla TEM、tet A、Sul2、aac(3)-Ⅱ、Par C。表明该牛场大肠杆菌分离株的耐药现象特别严重,提示该养殖场应合理使用抗生素。
In order to understand the drug resistance and drug-resistant gene transfer of diarrhea calf Escherichia coli isolates from a large-scale dairy farm in Suihua City, Heilongjiang Province, in April 2016, eight fecal samples of the diarrhea calf from the herd were collected, Isolation and identification, biochemical experiments and other identification methods to determine the cause of cattle diarrhea Escherichia coli infection. The results showed that they were highly sensitive to florfenicol and polymyxin B, and resistant to penicillin. The drug resistance rate was 100%. The resistance rates of tetracycline, ciprofloxacin and enrofloxacin reached 75 %. The resistance genes of Escherichia coli were detected by PCR, and five genes were identified as bla TEM, tet A, Sul2, aac (3) -Ⅱ, Par C. Indicating that the cattle Escherichia coli isolates resistant particularly serious, suggesting that the farm should be rational use of antibiotics.