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目的: 探讨β-胡萝卜素抑制白血病细胞增殖的机制。方法: 体外培养白血病细胞株HL-60,培养时添加10μm ol/L、50μm ol/L、100μm ol/Lβ-胡萝卜素作用24h、48h、72h 后,用台盼蓝拒染法进行活细胞计数,绘制细胞生长曲线;应用流式细胞术分析细胞周期分布,同时利用流式细胞术结合DNA 凝胶电泳检测白血病细胞凋亡。结果: 细胞生长曲线显示10~100μm ol/L的β-胡萝卜素均对白血病细胞株HL-60 有显著性抑制作用,且呈剂量效应关系。流式细胞术结果显示50μm ol/L的β-胡萝卜素处理HL-60 细胞24h 后,出现S期阻滞,并诱导5.59% 的细胞凋亡。DNA 凝胶电泳结果同样显示β-胡萝卜素能够诱导白血病HL-60 细胞凋亡。结论: β-胡萝卜素对于髓系白血病细胞株HL-60 细胞的增殖具有显著性抑制作用,其机制可能是干扰的白血病细胞DNA代谢;β-胡萝卜素诱导白血病细胞凋亡也是其抑制白血病的重要途径之一。
Objective: To investigate the mechanism of β-carotene inhibiting the proliferation of leukemia cells. Methods: In vitro leukemia cell line HL-60 was incubated with 10 μm ol/L, 50 μm ol/L, and 100 μm ol/L β-carotene for 24 h, 48 h, and 72 h, and then counted by trypan blue exclusion. The cell growth curve was plotted; cell cycle distribution was analyzed by flow cytometry, and apoptosis of leukemia cells was detected by flow cytometry combined with DNA gel electrophoresis. RESULTS: The cell growth curve showed that 10-100 μm ol/L β-carotene significantly inhibited the leukemic cell line HL-60 and showed a dose-effect relationship. Flow cytometry results showed that after treatment with 50 μm ol/L β-carotene for HL-60 cells for 24 h, S phase arrest occurred and 5.59% of cells were induced to undergo apoptosis. The results of DNA gel electrophoresis also showed that β-carotene can induce apoptosis in leukemic HL-60 cells. Conclusion: β-carotene has a significant inhibitory effect on the proliferation of myeloid leukemia cell line HL-60 cells. The mechanism may be the interference of DNA metabolism in leukemia cells; β-carotene induces leukemic cell apoptosis is also important in inhibiting leukemia. One of the ways.