目的阐明ＨＢＶ对感染细胞凋亡的影响。方法ＨｅｐＧ２及其转染ＨＢＶ的ＨｅｐＧ２２１５细胞培养中，加ＭＴＸ、ＡｃｔＤ或去血清培养，以ＦＣＭ检测细胞凋亡率。结果ＨｅｐＧ２．２．１５细胞加ＭＴＸ后２４小时和４８小时，凋亡率分别为１０８％和１３３％，加ＡｃｔＤ后分别为１６８％和３７７％，去血清后第４天及第６天，分别为１３２％和１４８％；而ＨｅｐＧ２细胞加ＭＴＸ后２４小时和４８小时，凋亡率则为１２６％和６５３％，加ＡｃｔＤ后分别为４４５％和８９７％，去血清后第４天和第６天凋亡率为１９８％和２８８％。结论在上述条件下，ＨｅｐＧ２２１５细胞较ＨｅｐＧ２细胞耐受凋亡；ＨＢＶ可能抑制肝癌细胞凋亡。 Objective To clarify the effect of HBV on the apoptosis of infected cells. Methods HepG2.2.2 cells transfected with HepG2 and their transfected HepG2 cells were cultured with MTX, ActD or de-serum, and the apoptosis rate was detected by FCM. Results After HepG2.2.15 cells were treated with MTX for 24 hours and 48 hours, the apoptotic rates were 108% and 133%, respectively. After addition of ActD, the apoptotic rates were 168% and 377% respectively. 4 days and 6 days, respectively, 13  2% and 14  8%; and HepG2 cells at 24 hours and 48 hours after MTX, the apoptosis rate was 12  6% and 65  3%, respectively, after addition of ActD 445% and 897%, respectively, and the apoptotic rates were 198% and 288% on the 4th and 6th day after the serum was removed. Conclusion Under the above conditions, HepG22.15 cells are more resistant to apoptosis than HepG2 cells and HBV may inhibit the apoptosis of HepG2 cells.