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鲎试剂检测热原时供试品的最适PH值为6.0～7.2,而葡萄糖注射液PH值为3.2～5.5,故应加以调节。临用时调PH易于污染,影响结果。因此,我们采用磷酸盐缓冲溶液代替生理盐水溶解鲎试剂。一、缓冲溶液的制备:取无水磷酸二氢钠0.40g,无水磷酸氢二钠0.47g,氯化钠0.46g,加适量蒸馏水使其溶解,再加入1克针用活性炭,最后加水至100毫升,煮沸30分钟,趁热用布氏漏斗过滤2～3次,再加蒸馏水至足量,分装于预先经180℃烘烤2小时的安瓿中,每支2毫升,于115℃灭菌30分钟即得。本品在无菌前后测PH值均为6.8左右,与葡萄糖注射液等量混合时PH仍不变。二、检测
鲎 reagent for pyrogen test for the best PH value of 6.0 to 7.2, and glucose injection PH value of 3.2 to 5.5, it should be adjusted. Pro use PH easy to pollute, affect the results. Therefore, we use a phosphate buffer solution instead of physiological saline to dissolve the reagent. First, the buffer solution Preparation: Take anhydrous sodium phosphate monobasic 0.40g, anhydrous sodium phosphate 0.47g, 0.46g of sodium chloride, add appropriate amount of distilled water to dissolve, then add 1 g of needle activated carbon, and finally add water to 100 ml, boiled for 30 minutes, filtered with hot Buchner funnel 2 to 3 times, plus distilled water to a sufficient amount, sub-packed in ampoules pre-baked at 180 ℃ for 2 hours, each 2 ml, at 115 ℃ off Bacteria 30 minutes that is. This product is measured before and after sterilization PH value of about 6.8, and glucose injection equivalent amount of PH injection is still the same. Second, testing