本文介绍一种可以自动读数的间接免疫荧光微量测定法,能简单、快速和客观地检测细胞表面和内部的抗原。悬浮小鼠巨噬细胞系GG2EE于磷酸盐缓冲液(PBS-Ⅰ),以2×10~5细胞/孔加入平底的96孔板,离心后弃去PBS-Ⅰ,用冷的、新鲜的戊二醛溶液(0.25%v/v PBS-Ⅰ),在40℃下固定孔内细胞5分钟,用PBS-Ⅰ洗涤3次,然后检测细胞表面抗原或内部抗原按稍有变动的标准间接免疫荧光染色程序进行,即: This article describes an indirect, indirect immunofluorescence micrometric assay that automatically reads antigens on the cell surface and inside simply, quickly and objectively. The mouse macrophage cell line GG2EE was suspended in phosphate buffered saline (PBS-I) and plated in flat-bottom 96-well plates at 2 × 10 -5 cells / well. After centrifugation, PBS-I was discarded and washed with cold, fresh, Dialdehyde solution (0.25% v / v PBS-I), fix the wells in the wells for 5 minutes at 40 ° C, wash them three times with PBS-I, and then detect standard cell surface antigens or internal antigens by standard indirect immunofluorescence Dyeing procedures, namely: