目的　观察人膀胱癌细胞 (BIU)在体外培养条件下的生长有序行为。方法　将BIU细胞悬液进行局限区域接种 ,待其形成贴壁生长的群落后进行宏观和显微动态观察及参数测量 (扩展直径、扩展速率、细胞形态、细胞平均密度、细胞平均大小、脱氢酶活性、对pH的敏感性等 )。结果　体外细胞培养条件下 ,局限区域生长的BIU细胞群落能出现明显的层次特性 ,不同区域细胞的形态、大小、密度、脱氢酶活性以及对pH的敏感性均存在差异。结论　空间封闭和生物耗散导致细胞自组织 ,其结果表现为生长有序行为。本实验为人体细胞的波动生长提供了一个简单可控的初步模型 Objective To observe the growth order of human bladder cancer cells (BIU) cultured in vitro. Methods BIU cells were inoculated into confined area, and macroscopic and microscopic dynamic observation and parameter measurement (expanded diameter, expansion rate, cell morphology, average cell density, average cell size, dehydrogenation Enzyme activity, sensitivity to pH, etc.). Results Under in vitro cell culture conditions, the BIU cell community growing in confined areas showed obvious gradation characteristics. The morphology, size, density, dehydrogenase activity and pH sensitivity of cells in different regions were different. Conclusions Space closure and bio-dissipation lead to cell self-organization, the results showed that the growth of orderly behavior. This experiment provides a simple and controllable preliminary model for the fluctuating growth of human cells