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目的初步探讨超声中药透入治疗促进大鼠股骨骨折愈合的疗效及其可能的作用机制。方法选择36只Sprague-Dawley成年大白鼠,建立股骨骨折模型,术后随机分为超声组、中药组、超声中药透入组和造模组。超声组于骨折部位行低强度超声治疗,中药组给予中药外敷治疗,超声中药透入组行超声中药透入治疗,造模组不给予任何治疗,在笼内自由活动。治疗30d后处死各组大鼠,取材后行HE染色及Ⅰ型胶原免疫组织化学染色。结果组织学检查结果显示:超声中药透入组的骨折端软骨性骨痂和骨性骨痂填充较其他各组明显,细胞与胶原纤维排列较其他各组有规律;免疫组织化学分析结果显示:超声中药透入组骨折端Ⅰ型胶原的表达较超声组和中药组强。结论中药超声透入促进骨折愈合的作用显著,较单纯中药或单纯超声治疗效果好;超声有促进中药透入的作用,其促进骨折愈合的机制有可能与促进Ⅰ型胶原的合成有关。
Objective To explore the effect of ultrasonic Chinese medicine penetration therapy on femoral fracture healing in rats and its possible mechanism. Methods Thirty-six adult Sprague-Dawley rats were used to establish a femoral fracture model. The fractures were randomly divided into an ultrasound group, a Chinese medicine group, an ultrasound penetration group and a model group. Ultrasound group underwent low-intensity ultrasound treatment at the fracture site. The Chinese medicine group was given topical application of traditional Chinese medicine. The ultrasonic Chinese medicine penetration group was treated with ultrasonic traditional Chinese medicine penetration. The model group received no treatment and was free to move within the cage. After 30 days of treatment, each group of rats was sacrificed and HE staining and type I collagen immunohistochemical staining were performed after harvest. Results The results of histological examination showed that the cartilage and osteophyte of the fracture end of the ultrasound-transplanted group were significantly more filled than those of other groups, and the arrangement of cells and collagen fibers was more regular than that of other groups. Immunohistochemical analysis showed that: The expression of type I collagen at the fracture end of the ultrasound-transplanted group was stronger than that of the ultrasound group and the TCM group. Conclusion Ultrasound penetration of traditional Chinese medicine promotes fracture healing significantly. It is better than simple Chinese medicine or ultrasound alone. Ultrasound can promote the penetration of traditional Chinese medicine. The mechanism of promoting the healing of fractures may be related to the promotion of type I collagen synthesis.