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采用反相高效液相法(PR-HPIC)对盐酸曲马多血浆药物浓度的测定方法及药物动力学进行了研究.实验应用Waters公司HPLC系统,WatersuBONDAPAK C18柱(4.6mm×150mm);测定采用内标峰高比定量,λ为216um.本方法最佳线性关系为r=0.9991,线性范围12.5～800ng/ml,最低检测浓度为6ng/ml,最低检测量0.12ng;方法平均回收率98.08%;浓度为800.00,200.00,50.00ng/ml的日内精密度分别为7.93,7.44,13.34,日间精密度分别为0.9,10.5,13.44.药物动力学参数分别为:T_（1/2） ka=0.51±0.23h,T_（1/2）β=5.92±2.57h,Tpeak=1.67± 0.61h,C_（max）=401.74±100.05ng/ml;AUCo_（→∞）=3242.92±1618.03(ng/ml)·h.
The method and pharmacokinetics of tramadol hydrochloride in plasma were studied by RP-HPLC.A WatersuBONDAPAK C18 column (4.6 mm × 150 mm) The internal standard peak-to-peak ratio was quantified, λ was 216um. The optimal linear relationship was r = 0.9991, the linear range was 12.5-800ng / ml, the lowest detection concentration was 6ng / ml and the lowest detection was 0.12ng. The average recovery was 98.08% ; The intra-day precision of the concentration of 800.00,200.00,50.00ng / ml were 7.93,7.44,13.34 respectively, the intra-day precision were 0.9,10.5,13.44 respectively.The pharmacokinetic parameters were T 1/2 (1/2) ka = 0.51 ± 0.23h, T 1/2 (1/2) β = 5.92 ± 2.57h, Tpeak = 1.67 ± 0.61h, C max = 401.74 ± 100.05ng / ml; AUCo → (→ ∞) = 3242.92 ± 1618.03 H