论文部分内容阅读
目的:探讨贝前列素钠对脑缺血再灌注大鼠血清白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)及髓过氧化物酶(MPO)的影响。方法:将雄性SD大鼠随机分为假手术组、模型组、贝前列素钠低剂量组、贝前列素钠中剂量组、贝前列素钠高剂量组。采用Zea Longa线栓方法造成大鼠局灶性脑缺血再灌注模型。术前30 min各组灌胃给药,贝前列素钠低、中、高剂量组分别给予贝前列素钠15,30,60μg·kg-1·d-1灌胃,对照组和模型组给予10 ml·kg-1·d-1的生理盐水。术后24 h处死大鼠制备血清,ELISA法检测各组血清IL-6、TNF-α水平及MPO活性。结果:贝前列素钠低、中、高剂量组大鼠血清IL-6、TNF-α水平及MPO活性明显低于模型组(P<0.05)。结论:贝前列素钠能够抑制大鼠脑缺血再灌注损伤时炎症细胞因子的表达,降低脑缺血再灌注损伤的程度。
Objective: To investigate the effects of beraprost on serum IL-6, TNF-α and myeloperoxidase (MPO) in rats with cerebral ischemia / reperfusion. Methods: Male SD rats were randomly divided into sham-operation group, model group, low-dose beraprost sodium group, middle-beraprost sodium group and high-dose beraprost sodium group. The model of focal cerebral ischemia-reperfusion in rats was induced by Zea Longa method. The rats in each group were intragastrically administrated 30 min before operation, and beraprost sodium (15, 30, 60 μg · kg-1 · d-1) 10 ml · kg-1 · d-1 of normal saline. The rats were sacrificed at 24 h after operation, and the levels of serum IL-6, TNF-α and MPO were measured by ELISA. Results: The levels of IL-6, TNF-α and MPO in the beraprost sodium low, middle and high dose groups were significantly lower than those in the model group (P <0.05). Conclusion: Beraprost can inhibit the expression of inflammatory cytokines in rats with cerebral ischemia-reperfusion injury and decrease the degree of cerebral ischemia-reperfusion injury.