molecularcloning相关论文
Structural Characterization and Molecular Cloning of Bioactive Peptides from the Skins of Amphibians
Amphibian skins have been used for centuries as components of traditional medicines due to their possession of a wide sp......
Molecular Cloning and Phylogenetic Analysis of Transformer-2 of the Oriental Fruit Fly Bactrocera do
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Neuron-specific enolase (NSE) is the key molecular marker for diffuse neuroendocrine system (DNES) cells,its expression ......
ACCase is the rate-limiting enzyme in plant storage lipid accumulation.Biotin carboxylase is an important subunit of ACC......
Molecular Cloning and Immune Function of a Delta-Class Glutathione Transferase in Chinese Mitten Cra
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MoleculaMolecular cloning and Expression Patterns of a Calmodulin Gene MCaM-3 From Mulberry (Morus L
Calmodulin (CaM) is a multifunctional Ca2 + -binding protein in eukaryotic cells,it plays important roles in regulating ......
Molecular Cloning and Function Analysis of the Defensin Gene in the Ixodid Tick,Dermacentor silvarum
Ticks are obligate,blood-feeding ectoparasites which usually parasitize mammals,birds and reptilia,and are among the mos......
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Phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) is the key enzyme of the general phenylpropanoid metabolic pathway.A ......
昆虫几丁质酶在害虫生物防治中具有很大的发展潜力。以甘蓝夜蛾Mamestra brassicae L.预蛹期幼虫整个虫体为材料提取总RNA,利用RT-......
参考大鼠EGFR基因序列，用RT-PCR方法对SD大鼠EGFR基因cDNA序列进行克隆，获得了SD大鼠EGFR基因的全长4127bp的cDNA序列（GenBank登录号H......
利用缺口修复（ｇａｐｒｅｐａｉｒ）方法克隆啤酒酵母（Ｓ．ｃｅｒｅｖｉｓｉａｅ）野生型ＲＡＤ２４基因，并将其亚克隆到Ｍ１３ｍｐ１８和Ｍ１３ｍｐ１９，用双脱氧末端终止法对该基因的两条链均进行了序列测定，ＤＮＡＳｔｒｉｄｅｒ程序分析显示该基......
本文以人胎盘全RNA为底物进行逆转录-聚合酶链反应(RT-PCR),制备出了人溶菌酶的cDNA片段。在限制性内切酶Sma Ⅰ存在的连接体系内,......
应用ＲＴ－ＰＣＲ方法，从新生大鼠脑组织总ＲＮＡ扩增大鼠ＦＭＲ１同源基因的ｃＤＮＡ片段，克降至ｐＵＣ１８质粒中进行序列分析。获得从终止密码子起共１６８１ｂｐ的编码序列，尚缺少约２００ｂｐ的５‘序列......
Cloning and characterization of porcine aquaporin 1 water channel expressed extensively in gastroint
AIM: To clone and characterize the porcine aquaporins (AQPs) in the gastrointestinal system.METHODS: A PCR-based cloning......
Identification of a chitinase from the hepatopancreas of Chinese black sleeper (Bostrychus sinensis)
Chinese black sleeper(Bostrychus sinensis)is a fish that lives both in seawater and freshwater,feeds on crustaceans,aqua......
Twenty cDNA differential fragments were isolated from the hippocampus of rats in epileptic state using mRNA differential......
Rat transforming growth factor β1 (rTGFβ1) cDNA from rat lymphocytes was cloned by RT-PCR and inserted into pcDNA3 to ......
目的克隆和研究支气管哮喘患儿白细胞介素-4(IL-4)近侧启动子区DNA序列的多态性. 方法取40名有明显家族及过敏史的哮喘患儿和10名......
本研究的目的是通过分子克隆、原核表达和蛋白纯化技术获得山羊GST-Sox2融合蛋白。用RT-PCR方法从山羊（Capra hircus）肠组织克隆了c-......
从含有鸡传染性支气管炎病毒（ＩＢＶ）的鸡胚尿囊液中快速提取病毒ＲＮＡ，得到约２３ｋｂ全长ＩＢＶ ＲＮＡ。运用反转录－聚合酶链反应（ＲＴ－ＰＣＲ）得到１．７２ｋｂ编码全长ＩＢＶ免疫原糖蛋白Ｓ１的基因片......
采用逆转录ＰＣＲ技术自ＳＤ大鼠脾细胞中克隆出全长１１２５个ｂｐ的编码主要组织相容性抗原Ｉ类分子（ＭＨＣ－Ｉ）ｃＤＮＡ，经限制性内切酶图谱分析证实后，定向插入表达载体ｐＢＶ２２０，并筛选......
目的 研究牛ＩｇＧＦｃ受体的结构与功能的关系。方法 在构建了牛肺巨噬细胞ＣＤＮＡ文库的基础上，用ＰＣＲ方法获得了码牛ＩｇＧＦｃ受体Ⅲ的全长编码基因。结果 通......
以提纯的我国标准ＵＤＶＦ４８Ｅ８强毒株基因组ＲＮＡ为模板、化学合成的融合蛋白（Ｆ）基因特异寡核苷酸为引物，反转录合成Ｆ基因ｃＤＮＡ，并采用同聚物加尾的方法克隆到细......
