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A simple cholesterol amperometric biosensor,based on in situ electropolymerization multi-wall carbon nanotubes-polyaniline nanocomposites and electrodeposited platinum nanoparticle films onto the surface of glassy carbon electrode,and then immobilizing cholesterol oxidase with adsorption effect and covalent interaction,was constructed in this paper.The assembled process of the modified electrode was probed by cyclic voltammetry,electrochemical impedance spectroscopy and scanning electron microscopy.Chronoamperometry was used to study the electrochemical performance of the resulting biosensor.The cholesterol biosensor exhibited performance with a linear range of 2.0×10-6-5.1×10-4 mol/L and a detection limit of 7.0×10-7 mol/L.The biosensor also showed a short response time (within 5 s).Moreover,the reproducibility,stability and interferences of the biosensor were also investigated.