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Aim: Gentiopicroside-containing extract and gentiopicroside from Gentiana manshurica Kitagawa (GM) was investigated for their hepatoprotective effects in vivo.Methods: We applied in vivo model of acetaminophen (APAP)-induced liver injury, D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced feminant hepatic failure and ethanol-induced acute alcohol liver injury models using C57BL/6 mice.