It has been established beyond doubt that giant panda genome lacks lignin-degrading related enzyme,gastrointestinal microbes may play a vital role in digestion of highly fibrous bamboo diet.However, there is not much information available about the intestinal bacteria composition in captive giant pandas with different ages.In this study,we compared the intestinal bacterial community of 12 captive giant pandas from three different age groups (subadults,adults, and geriatrics) through PCR-denaturing gradient gel electrophoresis (DGGE) and real-time PCR analysis.Results indicated that microbial diversity in the intestine of adults was significantly higher than that of the geriatrics (p < 0.05),but not significant compared to the subadults (P > 0.05).The predominant bands in DGGE patterns shared by the twelve pandas were related to Firmicutes and Proteobacteria.Additionally, in comparison to healthy individuals,antibiotic-treated animals showed partial microbial dysbiosis.Real-time PCR analyses confirmed a significantly higher abundance of the Lactobacillus in the fecal microbiota of adults (P < 0.05), while other bacterial groups and species detected did not significantly differ among the three age groups (P > 0.05).This study revealed that captive giant pandas with different ages showed different intestinal bacteria composition.