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A simple sample pretreatment for the determination of difenoconazole in cowpea was developed using micellar extraction combined with ionic liquid based vortex-assisted liquid-liquid microextraction(ME-IL-VALLME)prior to high performance liquid chromatography.In this method,the target analyte was seperated by surfactant tween 20 micellar solution,then the extract was diluted with pure water thus the concentration of surfactant was approached to its critical micelle concentration in extraction solution.The VALLME procedure was performed in the extraction solution by using 1-hexyl-3-methylimidazolium hexafluorophosphate([HMIM]PF6)as the extraction solvent and tween 20 as an emulsifier to enhance the dispersion of the waterimmiscible ionic liquid into aqueous phase.After vortexing and centrifugation,the fine droplet of ionic liquid was collected and diluted with methanol to HPLC analysis.Under the optimum conditions,the limits of quantitation was 0.1 mg kg-1 and good linearity with the correlation coefficients(γ)higher than 0.9984.The relative recoveries ranged from 64.0 to 81.3%with relative standard deviations of 7.9–9.6%.The porposed method provides more effective and green seperation procedure for cowpea in the micellar extraction step and high enrichment factor in VALLME step compared with conventional LLE or SPE.This ME-IL-VALLME method has a strong potential for the determination of fungicide in complex vegetable matrices.