【摘 要】
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This study aimed to develop a cell culture model of Huntington disease and observe the effect of sodium butyrate on this cell culture model.Exon 1 of both a wild type and a mutant IT15 gene from the g
【机 构】
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Department of Neurology, Affiliated Second Hospital, Zhejiang University School of Medicine, Hangzho
【出 处】
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中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
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This study aimed to develop a cell culture model of Huntington disease and observe the effect of sodium butyrate on this cell culture model.Exon 1 of both a wild type and a mutant IT15 gene from the genomic DNA of a healthy adult and a patient with Huntington disease,were amplified and cloned into the eukaryotic expression vector pEGFP-C1.Human neuroblastoma SH-SY5Y cells were transiently transfected with these recombinant plasmids in the absence and presence of sodium butyrate (0.1,0.2,0.5,1.0 mmol/L).The MTT assay is used to measure cell viability.The results indicated that the N-terminal fragment of mutant huntingtin forms perinuclear and intranuclear aggregates and causes a decrease in SH-SY5Y cell viability.Sodium butyrate inhibited the decrease in SH-SY5Y cell viability caused by the N-terminal fragment of mutant huntingtin.This suggests that sodium butyrate has a protective effect on this cell culture model of Huntington disease.
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