【摘 要】
:
目的 研究K141N HSPB8细胞内分布特点及41N HSPB8与HSPB1、NFL的共定位分析.方法 建立瞬时表达GFP-HSPB8和GFP-K141N HSPB8的SHSY-SY细胞、Hela细胞和HEK293T细胞模型,在激光共聚焦显微镜下观察GFP-K141N HSPB8聚集物形成情况,采用t检验和单因素方差分析的统计学方法分析聚集物形成的可能机理.运用间接免疫荧光染色进行GFP-K14
【机 构】
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中南大学湘雅三医院神经内科, 长沙410013 湘雅医院神经内科,长沙410008
【出 处】
:
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
目的 研究K141N HSPB8细胞内分布特点及41N HSPB8与HSPB1、NFL的共定位分析.方法 建立瞬时表达GFP-HSPB8和GFP-K141N HSPB8的SHSY-SY细胞、Hela细胞和HEK293T细胞模型,在激光共聚焦显微镜下观察GFP-K141N HSPB8聚集物形成情况,采用t检验和单因素方差分析的统计学方法分析聚集物形成的可能机理.运用间接免疫荧光染色进行GFP-K141N HSPB8与HSPB1、NFL的共定位分析.结果 K141NHSPB8形成以核周分布为主的聚集物.GFP-K141NHSPB8在不同内源性表达细胞系的聚集物形成百分率存在显著差异.GFP-K141N HSPB8与HSPB1、NFL存在免疫荧光共定位.结论 K141NHSPB8形成以核周分布为主的胞内聚集物,聚集物中K141N HSPB8与HSPB1、NFL存在共定位.聚集物形成的可能机理包括K141N HSPB8多肽链构象发生改变后出现自身异常聚集与家族内其他成员特别是HSPB1结合成异常的异源多聚体形成不可溶性大分子后产生聚集.NFs可能作为sHSPs的非特异性底物存在于聚合物中但并非聚合物形成所必需.
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