In the cement production process, human body contact and inhalation of cement, serious damage to the human digestive tract, the respiratory tract, great harm to health, so the national standard describes the limit of water soluble chromium (Ⅵ).This paper expounds the development of the reference material for water-soluble chromium(Ⅵ)content of cement, including sample preparation, test of homogeneity and stability, determination method, data processing and statistical uncertainty.The Certified Reference Material (CRM) is intended for the calibration of analytical instruments and the chemical analysis method of cement.It is also used in checking technical ability of chemical analysts.