巴氏灭菌后即食整肢小龙虾特定腐败菌的16S rRNA鉴定

来源 :第十届世界华人虾蟹养殖研讨会暨2016年中国河蟹产业发展高峰论坛 | 被引量 : 0次 | 上传用户:yan3134
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  对常规巴氏灭菌后即食整肢小龙虾(采用铝箔袋真空包装)的特定腐败菌(specific spoilage organism,SSO)进行了鉴定.采用传统分离纯化、菌落外形观察及细菌形态学镜检的方法,并结合16S rRNA分子生物学手段和限制性内切酶消化分析,结果共从样品中分离到82株典型菌,其中优势菌为苏云金芽孢杆菌(70株,占85.37%)和粘质沙雷氏菌(7株,占8.54%).本文首次分析了即食小龙虾SSO的菌相,可为后续加工相关产品时选择适宜的杀菌工艺提供理论依据,同时对今后设计合理的防腐保鲜剂配方也有重要指导意义.
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