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5. 乳腺癌细胞系ERα基因启动子甲基化与MTA1关系的研究

乳腺癌细胞系ERα基因启动子甲基化与MTA1关系的研究

来源 :第十八届全国临床肿瘤学大会暨2015年CSCO学术年会 | 被引量 : 0次 | 上传用户：hfutnyd

【摘 要】

：

目的：探讨乳腺癌细胞系中雌激素受体α(ERα)启动子甲基化状态的改变,对其MTA1 mRNA和蛋白表达水平的影响,研究ERα启动子去甲基化与MTA1表达的关系。方法：用去甲基化药物5-Aza-dC处理ERα启动子高甲基化状态的MDA-MB-435s和低甲基化状态的MDA-MB-231乳腺癌细胞系,MSP法检测ERα启动子甲基化状态,Western-blotting法检测MTA1蛋白的表达水平,RT

【作 者】

：

孙亚楠 毛晓韵 

【机 构】

：

沈阳 中国医科大学附属第一医院普外综合/烧伤 沈阳 中国医科大学大学附属一院乳腺外科

【出 处】

：

第十八届全国临床肿瘤学大会暨2015年CSCO学术年会

【发表日期】

：

2015年4期

【关键词】

：

乳腺癌 MTA1 雌激素受体α甲基化 

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　　目的：探讨乳腺癌细胞系中雌激素受体α(ERα)启动子甲基化状态的改变,对其MTA1 mRNA和蛋白表达水平的影响,研究ERα启动子去甲基化与MTA1表达的关系。方法：用去甲基化药物5-Aza-dC处理ERα启动子高甲基化状态的MDA-MB-435s和低甲基化状态的MDA-MB-231乳腺癌细胞系,MSP法检测ERα启动子甲基化状态,Western-blotting法检测MTA1蛋白的表达水平,RT-PCR法检测MTA1 mRNA的表达水平,对比2组细胞系中5-Aza-dC处理组及未处理组MTA1 mRNA和蛋白表达水平。

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