Backgroun: Several studies have shown that drug-eluting stents induce vascular endothelial dysfunction.These studies evaluated coronary vasomotion in the peri-stent coronary segment alone for one lesion with one drug-eluting stent.We investigated the angiographic and clinical characteristics of coronary spasm in patients with drug-eluting stents in acetylcholine provocation test at follow-up.Methods and results: All patients at our hospital who coronary angiography at follow-up after drug-eluting stents (sirolimus eluting Cypher and pactlitaxel-eluting Taxus stents) deployment between July 2007 and March 2009 were included.We performed an acetylcholine provocation test for diagnosing coronary vasospasm three vessels except for those with significant stenosis or contraindications to acetylcholine provocation or need of intoroglycelin for hemodynamic deteriolation druing initial provocation.Overall, the provocation test was positive 65.45% (36/55) of the coronary arteries and in 71.4% (30/42) of the patients with drug-eluting stents.There was no difference in the positive rate between patients with and without symptoms during follow-up period.A total of 65% of asymptomatie also showed positive results.In patients with positive results in Ach provocation test the vasoconstriction at distal segments to the stent was exaggerated compared with corresponding segments to in the non-stented vessels (0.46□ }0.27 vs 0.31 □ }0.20, P=0.008).In 36 vessels with positibe results spasm was provoked in the distal segments to the stent allone in 27 vessels.Vessels with positive results had a longer stent length compared with those with negative results (31.6□ } 13.6mm vs 24.2 □ } 11.2mm, P=0.0049).Conclusions: This study showed that drug-eluting stents might have the possibility to exaggerate the vasoconstriction by acetylcholine and total stent length was longer in vessels with positive results compared with those with negative results irrespective of the presence of symptom in the Japanese patients.