In our ongoing study on the medicinal plant project, we have produced MAbs against many bioactive component in TCM and applied them for ELISA, immunoaffinity chromatography, eastern blotting, immunocytoloealization and scFv formation.In this symposium we focus newly established eastern blotting and immunoaffinity concentration (knockout extract).Eastern blotting: Since small molecular compound like saponin can not conjugate to a membrane, no success of staining has been reported.We reached to a new idea that a saponin was divided into two functions, sugar and aglicone moieties.Sugar part can be oxidized to give aldehyde group which is fixed to membrane.Finally we succeeded to stain the aglycone part by MAb.Double staining by two MAbs indicated two coloring, blue and purple which distinguish aglycone differences.Knockout extract: Immunoaffinity column conjugated with MAb which is prepared via hydrazone conjugation with gel can be used for immunoconcentration resulting in high sensitive quantitative analysis of target compound even if low concentration, and makes it possible to isolate the target compound by one step.The washed out extract was named as knockout extract.We will discuss about how to use the knockout extract.