目的:探讨肿瘤微环境中PTEN对CCL2、VEGF及NHEFR1/EBP50继而对巨噬细胞表型转化影响.方法:用小鼠乳腺癌4T1培养细胞上清(33.3％稀释比例)培养驯化RAW264.7细胞24至72小时模拟肿瘤微环境.将驯化后的RAW264.7细胞通过小干扰RNA技术沉默PTEN基因,或是通过加入PI3K抑制剂LY294002提高PTEN的表达建立两个PTEN不同表达水平的细胞模型.采用RT-P
Improving the immunogenicity of HIV-1 envelope glycoproteins (Envs) is a challenge for vaccine development.Trimeric Envs may elicit specific binding antibodies (Abs) but less potent or broad neutraliz
Background: Two dendritic cell (DC) subsets are dependent on interferon regulatory factor 8 (IRF8): plasmacytoid DCs and conventional CD8+DCs.Granulocyte macrophage colony-stimulating factor (GM-CSF)
Background: F protein expressed from the HCV core coding sequence.The recent discovery of this novel F protein certainly challenges whether core is responsible for the various activities attributed to
Background Researchers reported a novel F protein, an alternative reading frame protein expressed from th HCV core coding sequence.It is found that F protein can induce humoral immunity in HCV infecte
Background.Th0 cells differentiate into Th1 or Th2 depending on multiple transcription factors acting on specific time points to regulate gene expression.Th1 7 cells, a subset of IL-17-producing T cel
Trim家族存在于所有多细胞的动物体内,是包含了较多蛋白的一个相当大的家族,在人类体内已经发现70余种.Trim家族成员有典型的三个结构域,这三个结构域从N端到C端的依次是锌指结构域(RING finger)、1个或者2个B-box、1个卷曲螺旋(Coiled-coil)结构域.此外,该家族还有一个可变的C-末端.Ring结构域一般始于N端10-20个氨基酸残基.在功能上,锌指结构域结构不同底物或
Background: Notch signaling involves T cell differentiation.However, little is known about Th17 polarization and its mechanistic process.Objective: The aim of this study was to identify genes influenc
研究背景:Id1分子是一种抑制分化分子,它含有HLH结构域,可以和同样含有HLH结构域的E蛋白形成异源二聚体,抑制E蛋白的转录活性,对细胞的生存、分化产生影响.在T细胞相关的研究中,对于Id分子和E蛋白的研究主要集中于淋巴细胞的发育及免疫记忆细胞形成,而关于其发挥功能的一系列机制,目前还比较模糊.本研究主要探讨Id1分子影响TCR信号通路机制及其生物学意义.
Objective: The aim of this study was to characterize the immunological features responsible for improved treatment responses in 77 patients with chronic hepatitis B (CHB) treated with peginterferon (P