Bisphenol A (BPA), an estrogenic compound, has been highlighted due to its endocrine disrupting actions on the human body, previous studies have shown that BPA may affect the male reproductive function.In this report, BPA at a dose of 100 μg/kg/day for 30 days via intragastric administration significantly decreased the epididymis weights, and increased the sperm abnormality rate, the histology of the testis showed unnormal.Similar to WT mice,the epididymis weights were decreased, the sperm abnormality rate was increased, and the histology of the testis showed unnormal in αERKO and βERKO mice after BPA treatment.Using real-time PCR, we observed that mRNA expression of ERαand ERβ was significantly changed in the testis and epididymis in BPA-treated animals, we also found that the BPA-induced mRNA expression of Cav3, RyRs and IP3Rs in testis and epididymis was dependent on ERs.Thus, it appears that BPA-induced male reproductive toxicology is mediated through the ERα and ERβ.However, the mode of action of BPA on the reproductive system and the potential mechanism of BPA affecting the male reproductive function require further investigation.