【摘 要】
：
(Objective)This experiment was conducted to evaluate immune effect between different domain of EtMic1 polypeptides displayed on yeast cells.In the present study,three different live oral vaccines usin
【机 构】
：
Department of Preventive Veterinary Medicine,College of Veterinary Medicine,Shandong Agricultural Un
【出 处】
：
中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会
论文部分内容阅读
(Objective)This experiment was conducted to evaluate immune effect between different domain of EtMic1 polypeptides displayed on yeast cells.In the present study,three different live oral vaccines using the EBY100/pCTCON-2 yeast surface display system with different Eimeria tenella microneme-1(EtMic1)protein domain recombination were constructed and their protective efficacies against homologous challenge were compared by evaluating the body weight gains,relative growth rate,cecal lesion scores,oocyst output,oocyst decrease ratio,anti-coccidial index,the serum antibody levels and the proliferation ability of blood lymphocytes.(Result)The results indicated that all the three constructed live oral vaccines expressing different EtMic1 polypeptides provided excellent protection against homologous challenge by significantly increasing weight gains,reducing cecal lesions,achieving a high ACI,elevating specific antibody response and splenocyte proliferation ability compared with controls.(Conclusion)The yeasts displaying the EtMic1 polypeptide-Ⅲ(expressed TSP-2,TSP-3 and TSP-4 domains)provided significant better protection against challenge than the yeasts displaying either the EtMic1 polypeptide-Ⅰ(expressed Ⅰ-domain,TSP-1 and TSP-2)or polypeptide-Ⅱ(expressed Ⅰ-domain and all the five TSP domains)did.Considering the exclusion of antibiotic resistant gene in the system,the strain EBY100 of Saccaromyces cerevisiae may be a better choice for coccidian antigen delivery.
其他文献
(目的)鸡球虫生活史复杂,分为有性生殖阶段和无性生殖阶段,生活周期中蛋白表达谱有较大差异,其单一蛋白的基因工程疫苗仅具有部分保护力。因此本研究旨在构建鸡柔嫩艾美耳球虫不同抗原蛋白部分基因真核表达质粒以期为鸡球虫串联多表位活载体疫苗的研究奠定基础。(方法)利用基因功能预测软件,对已报道的具有较好免疫原性的Et MIC 2、Et MIC 3、EtAMA 1进行抗原表位的预测,截选抗原表位集中的基因片段
顶复门原虫作为专性胞内寄生病原,其逸出过程作为两次胞内周期的连接点对于本门原虫的生存和繁殖具有举足轻重的作用.因此,对该过程的研究有助于了解这类寄生原虫的致病机理,为开发新型有效的抗寄生虫药物提供科学依据.刚地弓形虫作为顶复门原虫的模式生物,有关其逸出过程的研究相对成熟.目前已发现并研究了多种与虫体逸出相关的蛋白,如：TgNHE1、TgPLP、TgCDPK1等.然而对于本门的另外一种重要病原艾美耳
本研究旨在克隆柔嫩艾美耳球虫(Eimeria tenella)5-磷酸脱氧木酮糖还原异构酶(1-deoxy-D-xylulose-5-phosphate reductoisomerase,DXR)基因,并初步分析EtDXR的酶活性.根据EuPathDB数据库信息注释、拼接Etdxr基因序列,根据注释序列设计特异性扩增引物,以E.tenella第二代裂殖子总RNA为模板,RT-PCR方法扩增Etdx
兔球虫是危害家兔健康养殖的重要病原之一,转基因技术是深入研究球虫细胞生物学、球虫与宿主相关作用及其开发用做真核表达载体的重要工具.本研究以兔肠艾美耳球虫(Eimeria intestinalis)弱毒株为模式兔球虫,通过子孢子电转染、无球虫兔体内传代和流式分选等系列试验方法和技术,首先建立了兔球虫稳定电转染体系,并获得了稳定表达YFP的转基因E.intestinalis虫株,转基因球虫卵囊在第三次
在分析了多种艾美耳球虫18S rDNA序列的基础上,设计一对艾美耳属球虫(Eimeria)的通用引物,分别对鹅艾美耳球虫(E.anseris)、赫尔曼艾美耳球虫(E.hermani)、多斑艾美耳球虫(E.stigmosa)和金黄艾美耳球虫(E.fulva)18S rDNA部分序列进行PCR扩增、克隆和测序,并用DNAstar软件对这4种鹅球虫与1 5种艾美耳球虫的18S rDNA序列进行同源性分析
Eimeria parasites as importantly obligate intracellular apicomplexan protists can cause coccidiosis,resulting in high economical loss in the poultry industry annually.Genes function analysis of potent
Avian coccidiosis,caused by the infection of seven Eimeria spp,can cause huge economic loss in poultry industry.Eimeria maxima and Eimeria tenella are major pathogenic species as well as extreme immun
The search for effective vaccines against chicken coccidiosis remains a challenge because of the complex organisms with multiple life cycle stages of Eimeria.Combination ofT-cell epitopes from differe
The CD40 ligand(CD40L)has shown potential as a powerful immunological adjuvant in various studies.Here,the efficacy of a chimeric subunit vaccine,consisting of Eimeria tenella immune mapped protein 1(
Avian coccidiosis is mostly caused by mixed infection of several Eimeria species under natural conditions and immunity to avian coccidiosis is largely dependent on T-cell immune response.In this study