Gold nanoparticles (AuNPs),possessing good optical property,high surface energy,low toxicity and excellent attachment to molecules of biological interest [1],are now of intense interests in scientific applications,such as optics,catalysis,biodiagnostic systems,photothermal and targeted drug delivery treatment [2,3].In this present work,based on the optimum catalytic effect of AuNPs (size of 13 nm,particle number of 1.81×1010 L-1) on luminol chemiluminescence (CL) reaction,the luminescence behavior of luminol-AuNPs system with bovine serum albumin (BSA) was first investigated by flow injection (FI) CL analysis.It was found that BSA can weaken the enhancement of AuNPs on luminol CL reaction,showing ΔI2 (Ilu-AuNPs-BSA-Ilu-BSA)< ΔI1(Ilu-AuNPs-Ilu-O2).It was also found that the relative CL intensity were linear with BSA concentrations in the range of 1.0×10-10~ 1.0×10-8M,yielding calibration equation of ΔICL=7.57CBSA-7.50(R = 0.9975,ΔICL = Ilu-AuNPs-BSA-Ilu-AuNPs) and a detection limit of 3.0×10-11 M (3σ).The possible CL mechanism of luminol-AuNPs-BSA reaction was studied in the processing work.