【摘 要】
：
目的:研究峨眉桃叶珊瑚果实中桃叶珊瑚苷的提取纯化工艺.方法:以提取率为指标,采用单因素法考察峨眉桃叶珊瑚果实中桃叶珊瑚苷醇提工艺;以纯度和回收率为指标,考察桃叶珊瑚苷的硅胶柱色谱法及结晶法纯化工艺.结果:桃叶珊瑚苷的最佳提取条件:以80％乙醇为溶剂,料液比1:10,提取温度80℃,回流提取3次,1.0h/次;硅胶柱色谱纯化工艺:以甲醇-氯仿(1∶5和1∶3)梯度洗脱,洗脱剂用量为3 BV,柱子径高
【机 构】
：
西南交通大学生命科学与工程学院,四川 成都 610031 清远嘉博制药有限公司,广东 清远 511
【出 处】
：
中华民族医药资源保护与开发应用高峰论坛
论文部分内容阅读
目的:研究峨眉桃叶珊瑚果实中桃叶珊瑚苷的提取纯化工艺.方法:以提取率为指标,采用单因素法考察峨眉桃叶珊瑚果实中桃叶珊瑚苷醇提工艺;以纯度和回收率为指标,考察桃叶珊瑚苷的硅胶柱色谱法及结晶法纯化工艺.结果:桃叶珊瑚苷的最佳提取条件:以80％乙醇为溶剂,料液比1:10,提取温度80℃,回流提取3次,1.0h/次;硅胶柱色谱纯化工艺:以甲醇-氯仿(1∶5和1∶3)梯度洗脱,洗脱剂用量为3 BV,柱子径高比为1∶15,流速为2 BV·h-1,上样量35 mg·mL-1;结晶工艺:以正丁醇为结晶溶剂,加热沸腾使样品完全溶解,40℃保温过滤,滤液减压回收正丁醇至20倍量,低温搅拌析晶2h,得粉末状晶体后,低温冷冻离心3次,取下层真空干燥即得桃叶珊瑚苷精制品,其纯度与回收率的平均值约为93.07％与70.94％.结论:该工艺可较好提取纯化峨眉桃叶珊瑚果实中桃叶珊瑚苷.
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