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Researches have proved that spices have some bioactive functions,and the common function is antibacterial activity.The antibacterial activity to inhibit the growths of Listeria monocytogenes of essential oils (EOs),extracted from the spices of cinnamon (Cinnamomum cassia Presl),laurel (Laurus nobilis L) and clove (Syzygium aromaticum,syn.),were different in our previous study.The headspace solid-phase micro extraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) techniques were used to determine components of the EO.Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to examine the ultrastructural changes in bacteria.