目的：使用不同方法对蛋白质的核质分布进行检测并比较差异。方法 细胞经免疫荧光染色后使用高内涵分析对蛋白质的细胞质或细胞核总荧光强度进行分析；分别提取胞浆及胞核蛋白,使用Western Blot对核质组分中的蛋白质含量进行鉴定。结果 两种方法均可检测到蛋白质的出核转运；经定量分析,HCA法检测到的HuR蛋白质/核比升高倍数小于WesternBlot法。结论 使用高内涵分析检测蛋白质核质定位具有准确、
帕金森病(Parkinsons Disease,PD)目前是中老年人群发病率排第二的神经退行性疾病,但PD的发病机制尚不完全清楚,主要病理学特征是晚期可见中脑多巴胺能神经元死亡,残存的细胞中存在以α-突触核蛋白(α-synuclein,α-syn)为主要成分的路易氏体(Lewy bodies,LBs)。α-syn作为第一个被发现的PD致病基因,在PD发生发展过程中起到重要作用,尽管有研究发现α-s
Herein,we report a biomimetic method to synthesize needle-like calcium phosphate (CaP) with a dimension of ~130 nm in length and ~ 30 nm in width by using carbon dots (CDs) and sodium carboxymethylcel
Purpose: Zinc-doped copper oxide nanocomposites (nZn-CuO NPs) are novel nanoparticles synthesized by our group.In the present study,the antitumor effects and the underlying molecular mechanisms of the
Recent studies have indicated that urotensin Ⅱ (UII)is involved in insulin resistance.We investigated the effects of UII on hepatic insulin resistance and the role of nicotinamide adenine dinucleotide
Dengue virus (DV) has four distinct serotypes.A proper dengue vaccine should be tetravalent and induce equivalent and durable specific immune responses against all serotypes simultaneously.There are t
Parkinsons disease (PD) is the second most common neurodegenerative disorder,but there are few treatments currently available.The autophagy pathway plays an important role in the pathogenesis of PD;mo
Objectives: To generate a truncated soluble IL-17RC protein to ameliorate symptoms of inflammation and bone destruction through IL-17A and IL-17F neutralization.Methods: The exons constituting the IL-
Parkinsons disease (PD) is the second most common neurodegenerative disorder after Alzheimers disease.It is caused by genetics,environmental factorsand aging,while there are currently few treatments a
Olfactory impairment is one of the earliest non-motor manifestations of Parkinsons disease,which is accompanied by abnormal α-synuclein (α-syn) deposition in olfactory neurons.However,the mechanistic