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Colorectal cancer is one of the leading causes for tumor-associated death and traditional Chinesemedicine (TCM) classifies colorectal cancer into various subtypes mainly according to thesymptomatic pattern identification (ZHENG).Here we investigated the difference in metabolic profiles of serum bycomparing colorectal cancer subjects with Non deficiency (ND),Qi deficiency (QD) and Yin deficiency (YD).A total of 90 patients with colorectal cancer enrolled in this study were distributed as ND (N=30),QD (N=30) and YD (N=30).Before serum metabohc analysis,anthropometric parameters and symptom index were measured.Metabolomics profiling was performed by using gas chromatography-mass spectrometry (GC-MS).The ratio of subjects with carcino-embryonic antigen (CEA) was higher in YD pattern,and the ratio of subjects with carbohydrate antigen 19-9 (CA19-9) was higher both in YD and QD,compared with ND.As a result of metabolomics analysis,twenty-five metabolites displayed differences between QD and ND,while twenty-eight metabolites displayed differences between YD and ND.The down-regulated metabolites in QD/ND and YD/ND mainly includes carbohydrates and the up-regulated metabolites mainly includes amino acids and fatty acids,suggesting conversionobstruction of carbohydrates,fatty acids and amino acidsoceurs in patients with QD and YD compared with ND.Our results demonstrate thatcolorectal cancer patients with QD or YD were associated with metabolic disorders and the variations of serum metabolic profiles may serve as potential biochemical markers for diagnosis and prognosis of colorectal cancer patients displayed QD or YD patterns.