Autopolyploidy is widespread in higher plants and plays an important role in the process of evolution.In this study,autotetraploidy was induced by colchicine in Chrysanthemum lavandulifolium.Ligulate flower,tubular flower and leaves of tetraploid plants were greater than of the diploid plants,and leaves of them was thicker and larger resulting from the increase in number and size of cells.Diploid and tetraploid plants were used to investigate the genomic,transcriptomic and epigenetic changes in the early stages of autopolyploidy formation.The genomic variation followed autopolyploidization was investigated using sequence-related amplified polymorphism(SRAP),and DNA methylation variations were detected by methylation-sensitive amplified polymorphism(MSAP)technologies.Transcriptomic changes were detected via cDNA fragment length polymorphism(cDNA-AFLP).