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(-)-Epigallocatechin-3-gallate (EGCG), a major polyphenol component of green tea, has recently been identified as an inhibitor of hepatitis C virus (HCV) entry.Here, we examined whether EGCG can enhance hepatocyte-mediated intracellular innate immunity against HCV.HCV dsRNAs (Core, E1-P7,NS-3'NTR and NS5A) induced interferon-λ 1 (IFN-λ 1) expression in human hepatocytes.These HCV dsRNAs also induced the expression of toll-like receptor 3 (TLR3), retinoic acid-inducible gene Ⅰ (RIG-Ⅰ)and several antiviral IFN-stimulated genes (ISGs) expression.Although EGCG treatment of hepatocytes alone had little effect on TLR3/IFN signaling pathway, EGCG significantly enhanced HCV dsRNAs-induced the expression of IFN-λ 1, TLR3, RIG-Ⅰ and antiviral ISGs in hepatocytes.Furthermore, treatment of HCV-infected hepatocytes with EGCG and HCV dsRNAs inhibited viral replication.EGCG has the ability to enhance HCV dsRNAs-induced intracellular antiviral innate immunity against HCV infection.These data suggest the potential application of EGCG as a new antiviral agent for HCV therapy.