【摘 要】
：
Somatic cell nuclear transfer (SCNT) has been widely used in the generation of transgenic pigs, but the cloning efficiency is still needed to be largely improved.Incomplete epigenetic reprogramming an
【机 构】
：
Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary
论文部分内容阅读
Somatic cell nuclear transfer (SCNT) has been widely used in the generation of transgenic pigs, but the cloning efficiency is still needed to be largely improved.Incomplete epigenetic reprogramming and abnormal gene expression during early embryonic development was considered to be attributed to such low efficiency.It has been shown that a low-toxicity histone deacetylase inhibitor Scriptaid had positive effect on affecting the developmental competence of porcine SCNT embryos to improve the cloning efficiency.In the present study, it was also found that the blastocyst formation rate of embryos with 500 nM Scriptaid treatment for 15 hafter activation was enhanced compared to the control (27.7% vs.12.2%, p<0.05).
其他文献
Klf4 is a transcription factor in the family of Kruppel-like factors and plays important roles in stem cell biology, however, its function during embryogenesis is unknown.Here, we report the character
BCAS2 gene is initially identified as an up-regulated gene in MCF-7 and BT-20 breast cancer cell and one of the core component in CDC5L/PRP19 complex, which are an evolutionarily conserved splicing co
The Wnt signaling pathway has been reported to play essential roles in embryonic patterning among all the examined metazoans.Recent studies on Wnt genes in the basal chordate amphioxus embryogenesis h
Although maternal effect genes play critical roles in early embryogenesis, their molecular functions in mammals remain largely unknown.Using mouse genetics and biochemistry, we have identified a mamma
合子基因组激活之前,动物早期胚胎的RNA转录活性很低,RNA的代谢主要表现为母源RNA的降解,功能RNA的剪切活性很低,随合子基因组激活,RNA的剪切活性明显增强.通过RNA剪切体加工前体RNA是所有真核生物的重要基因表达调控方式之一,前体RNA在哺乳动物早期胚胎细胞中加工的分子机制目前仍然不清楚.BCAS2 (Breast cancer amplified sequence 2)最初在研究RNA
Zona pellucida is the glycoprotein coat surrounding the oocyte and early embryo, which is known to be crucial to the oocyte maturation and fertilizaiton.Mouse zona pellucida is made from three compone
The development of mammalian spermatozoa relies on coordinated regulation of gene expression, protein synthesis and translocation of sperm components into right places at right time during spermatogen
Phosphorus (P) is one of essential nutrient elements for plant growth and development.Although abundant P is present in a lot of soils, very little of it is present in phosphate (Pi) forms that are av
Ex vivo organ culture is a powerful technique for studying kidney development for last fifty years and it recapitulates many aspects of early renal development very well.Due to the three-dimensional n
Mammalian genome is transcribed into long and short non-coding RNAs (ncRNAs) in a developmentally regulated pattern.Long ncRNA (lncRNA) was defined as more than 200nt ncRNA, although the molecular fun