稀释法抗核抗体的测定及其临床应用价值探讨

来源 :中国免疫学会第九届全国免疫学学术大会 | 被引量 : 0次 | 上传用户：A3512516

【摘要】

：

【作者】

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王广杰刘辉

【机构】

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大连医科大学附属第一医院大连医科大学

【出处】

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中国免疫学会第九届全国免疫学学术大会

【发表日期】

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2014年8期

【关键词】

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抗核抗体酶联免疫吸附法标本稀释法

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目的:利用标本稀释法,采用酶联免疫吸附技术和间接免疫荧光技术检测抗核抗体,判断该方法诊断自身免疫性疾病(AID)优越性和诊断价值.方法:将155例研究对象分为四组:Ⅰ组:SLE组33例;Ⅱ组:其他AID组的59例;Ⅲ组:非AIDs组43例;Ⅳ组:健康对照组20例.采用酶联免疫吸附抗核抗体谱(EIA-ANA)法检测各组研究对象的自身抗体,同时用间接免疫荧光法(IIF-ANA)测定各组的抗核抗体(ANA).凡是高于最低检测线的结果需要稀释,通过公式计算测定结果来探讨临床应用价值.利用SPSS11.5统计软件进行统计分析.多组间比较利用方差分析,两种方法的相关性采用Spear相关性分析.

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稀释法抗核抗体的测定及其临床应用价值探讨

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