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Dermatomyositis (DM)is an autoimmune inflammatory myopathy (IM) primarily affecting muscle and skin.The underlying mechanisms of DM remains poorly understood.This study aimed to explore the change of gene expression profile, investigate the underlying mechanism and identify new targets for DM.The data GSE48280, including 5 DM and 5 normal DM muscle tissue samples were available from Gene Expression Omnibus.Firstly, differentially expressed genes (DEGs) were screened by Limma package in R.Subsequently, functional and pathway enrichment analysis were performed using ClueGO from Cytoscape.Finally, the protein-protein interaction (PPI) network was constructed by STRING and Cytoscape to identify hub genes.As a result, a total of 180 up-regulated and 21 down-regulated genes were identified.The GO enrichment analysis revealed the type Ⅰ interferon signaling pathway was the most significant term enriched with the DEGs.Through the KEGG pathways analysis, we obtained 27 significantly pathways and most of them can be divided into infectious diseases and immune system categories.After the PPI network constructed, 24 hub genes were selected, all of which involved in type Ⅰ interferon signaling pathway in DM.Our findings supported that type Ⅰ interferons (IFNs) play a central role for the induction of DM muscle disease.In addition,the DGEs of CCL5, CXCL10, TLR3, DDX58, IFIH1, ISG15 and Mx1 may be used as potential targets for DM diagnosis and treatment.