Microfluidic techniques open up new opportunities for manipulating the living environment of micron-sized organisms,such as Drosophila embryo,zebra fish and nematode,at high-resolution.In this work,a microfluidic system capable of developing a Drosophila embryos anterior and posterior halves controlled at different temperature was established to study the embryos reaction under environmental perturbations.The microfluidic device was coupled to a confocal microscopy for real-time image acquisition.It was found that the embryo can counteract the effects of these unnatural environmental conditions within each development cycle.