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In the thesis,we synthesized three 20mer caged circular asODNs(R20,R20B2 and R20B4) with a photocleavable linker and an amide bond linker between two 10mer ODNs.With these caged circular asODNs,RNA binding affinity and its digestion by RNase H were readily photomodulated.RNA cleavage rates were upregulated about 43-,25-and 15-fold for R20,R20B2 and R20B4,respectively,upon light-activation in vitro.