【摘 要】
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Objectives: To investigate whether the miR-21 could down-regulate FASLG to regulate the proliferation and apoptosis in glioblastoma cancer stem cells(GSCs).Methods: Real-Time quantitative PCR was appl
【机 构】
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Department of Neurobiology,College of Basic Medicine,China Medical University,Shenyang,110001,China
【出 处】
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第一届辽宁省神经科学学会学术研讨会
论文部分内容阅读
Objectives: To investigate whether the miR-21 could down-regulate FASLG to regulate the proliferation and apoptosis in glioblastoma cancer stem cells(GSCs).Methods: Real-Time quantitative PCR was applied to evaluate the expression level of miRNA-21 in glioblastoma and normal brain tissue samples,and the expression change of miRNA-21 between GSCs and non-GSCs was also detected.The proliferation and apoptosis ability of miR-21 in GSCs were analyzed by MTT and flow cytometry assay after pre-miR-21 or anti-miR-21 transfection.For the regulation mechanism analysis of miR-21,TargetScan,PicTar and miRBase were used to predict the possible target gene of miR-21.Luciferase activities assay and western blot were used to reveal the predicted target gene of miR-21 were direct and specific.RNA interference technology was used to confirm the proliferation and apoptosis regulation of miR-21 was directly induced by FASLG.
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