染色体畸变不仅能导致严重的遗传性疾病,而且与致癌作用有关,因而化学物引起对染色体的畸变效应已被认为是预测化学物致癌性的重要指标。检测染色体畸变的细胞遗传学方法随所用组织和动物种的不同而各异,但经典的方法是分析有丝分裂或减数分裂的中期细胞。虽然制备染色体标本并不复杂,但分析标本却较为困难且颇费时间。由于获得的中期细胞数较少,其统计效能往往偏低。而检测子代细胞群中微核出现率却是评价靶细胞染 Chromosomal aberrations not only lead to serious genetic diseases, but also to carcinogenic effects, and thus chemicals caused by chromosome aberration effect has been considered to predict chemical carcinogens important indicator. Cytogenetic methods for detecting chromosomal aberrations vary with the tissue and animal species used, but the classic method is to analyze mitotic or meiotic metaphase cells. Although the preparation of chromosome specimens is not complicated, the analysis of specimens is more difficult and time-consuming. Due to the small number of metaphase cells obtained, their statistical performance tends to be low. However, the detection rate of micronuclei in the progeny cell population is the target cell staining