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目的:观察异丙酚(propofol,PPF)预处理对心肺复苏后大鼠学习记忆能力的影响以及海马神经元的保护作用。方法:将80只Sprague-Dawley大鼠随机分为正常对照组、复苏+英脱利匹特对照组(R+intralipid)、复苏+异丙酚低剂量预处理组(R+PPF 10 mg/kg)、复苏+异丙酚高剂量预处理组(R+PPF 50 mg/kg)。实验组窒息前10 min腹腔注射10%异丙酚10 mg/kg或50 mg/kg。建立大鼠心肺复苏模型,并在复苏后3 d进行Morris水迷宫检测大鼠学习记忆能力,海马Nissl染色观察神经元损伤情况,Western blot检测caspase-3的表达变化。结果:与正常对照组大鼠相比,复苏后大鼠出现明显的学习记忆能力的降低(P<0.05),异丙酚预处理则能显著改善复苏导致的行为学改变(P<0.05)。Nissl染色结果表明,心肺复苏后大鼠海马CA1区神经元排列稀疏、紊乱,细胞间隙增大。异丙酚预处理(10 mg/kg或50 mg/kg)后,神经元的数量和排列模式均有显著改善。Western blot结果显示心肺复苏后,海马caspase-3的表达显著升高。而异丙酚预处理(10 mg/kg或50 mg/kg)组caspase-3的表达与英脱利匹特对照组相比显著降低(P<0.05)。结论:异丙酚预处理可能通过改善心肺复苏后的神经元损伤而发挥脑保护作用。
OBJECTIVE: To observe the effect of propofol (PPF) pretreatment on learning and memory in rats after cardiopulmonary resuscitation and the protective effects of hippocampal neurons. Methods: Eighty Sprague-Dawley rats were randomly divided into normal control group, R + intralipid group and R + PPF 10 mg / kg group ), Resuscitation + propofol high-dose pretreatment group (R + PPF 50 mg / kg). The experimental group was injected intraperitoneally with 10% propofol 10 mg / kg or 50 mg / kg 10 min before asphyxiation. The cardiopulmonary resuscitation model was established in rats. Morris water maze was used to detect the learning and memory abilities of rats 3 days after resuscitation. Nissl staining in hippocampus was used to observe the neuronal injury. Western blot was used to detect the expression of caspase-3. Results: Compared with the normal control group, the recovery of rats showed obvious learning and memory abilities (P <0.05). Propofol pretreatment significantly improved the behavioral changes induced by resuscitation (P <0.05). Nissl staining results showed that after CPR, neurons in CA1 area of rat hippocampus were sparsely arranged and disorderly, and the intercellular space increased. Propofol pretreatment (10 mg / kg or 50 mg / kg), the number of neurons and arrangement patterns were significantly improved. Western blot results showed that after CPR, the expression of caspase-3 in hippocampus increased significantly. However, the expression of caspase-3 in propofol pretreatment group (10 mg / kg or 50 mg / kg) was significantly lower than that in the control group (P <0.05). CONCLUSION: Propofol preconditioning may play a neuroprotective role by improving neuronal damage after cardiopulmonary resuscitation.