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为发展新的快速产前诊断染色体非整倍体的方法，我们利用已发表的染色体α-卫星序列，设计特异性引物，应用原位引物标记技术检测了13、18、21号染色体。研究结果显示这是一种快速，简捷的染色体数目检测方法，蛋白酶消化处理可提高标记信号强度，准确掌握退火温度是保证标记信号特异性的关键，如何在原位标记过程中保持良好的染色体形态则有待于进一步改进实验方法。
To develop a new method for rapid prenatal diagnosis of chromosomal aneuploidy, we designed specific primers using published sequences of α-satellite chromosomes and detected chromosomes 13, 18 and 21 using in situ primer labeling. The results show that this method is a rapid and simple method for detecting the number of chromosomes. Protease digestion can increase the signal intensity of the labeled signal. Accurate understanding of the annealing temperature is the key to ensure the specificity of the labeled signal. How to maintain the good chromosomal status during in situ labeling It needs to further improve the experimental method.