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目的:研究结核分枝杆菌耐链霉素和乙胺丁醇的rpsL和emb B基因突变情况,探讨耐药基因突变与耐药性的关系。方法:通过传统药敏实验和聚合酶链反应(PCR)--单链构象多态性(SSCP)技术初步鉴定62株临床分离株的药敏和rps L、emb B基因。结果:与结核菌标准株H37Rv对照,分析30例TB菌耐链霉素(SM)的rps L基因,发现其突变率为70.0%(21/30),分析29例耐乙胺丁醇(EMB)的emb B基因,该基因的突变率为65.5%(19/29)。结论:部分结核分枝杆菌耐SM和EMB是由于其rps L、emb B基因突变所致,PCR-SSCP银染技术可能成为测定部分结核分枝杆菌耐药的简便、快速的方法。
OBJECTIVE: To study the mutations of rpsL and emb B in Mycobacterium tuberculosis resistant to streptomycin and ethambutol, and to investigate the relationship between drug resistance mutations and drug resistance. Methods: The drug susceptibility and rps L, emb B genes of 62 clinical isolates were preliminarily identified by traditional susceptibility test and polymerase chain reaction (PCR) - single strand conformation polymorphism (SSCP). Results: Compared with the standard strain of Mycobacterium tuberculosis H37Rv, the rps L gene of streptomycin (SM) in 30 TB strains was analyzed. The mutation rate was 70.0% (21/30) ) Emb B gene, the gene mutation rate was 65.5% (19/29). Conclusion: Some M. tuberculosis resistant to SM and EMB were caused by mutations of rps L and emb B genes. PCR-SSCP silver staining may be a simple and rapid method to determine the resistance of some M. tuberculosis strains.