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目的:探讨脑灵汤对阿尔茨海默病(AD)模型大鼠海马CA3区iNOS蛋白表达的影响,初步揭示脑灵汤可能的作用机制。方法:选取40只SPF清洁级SD大鼠,随机分为正常对照组、假手术组、AD组、脑灵汤组和脑复康组,每组8只,喂养1周后,采用Aβ1-42注射大鼠海马法制成大鼠模型,再喂养28 d,免疫组织化学法观察iNOS蛋白在大鼠海马CA3区中的表达。结果:AD组大鼠海马CA3区iN-OS蛋白表达较正常对照组增多(P<0.05),脑灵汤治疗后iNOS蛋白的表达降低。结论:脑灵汤能明显改善AD模型海马CA3区iNOS表达,对老年性痴呆大鼠有治疗作用。
Objective: To investigate the effect of naoling decoction on the expression of iNOS protein in hippocampal CA3 region of Alzheimer disease (AD) model rats and to reveal the possible mechanism of Naoling decoction. Methods: Forty Sprague-Dawley (SD) SD rats were randomly divided into normal control group, sham operation group, AD group, Naoling decoction group and naofukang group. Each group was fed with Aβ1-42 The rats were injected into the hippocampus of rats to establish a rat model for 28 days. The expression of iNOS protein in hippocampal CA3 region of rats was observed by immunohistochemistry. Results: The expression of iN-OS protein in hippocampal CA3 region of AD group was higher than that of normal control group (P <0.05). The expression of iNOS protein was decreased after treatment with Naoling decoction. Conclusion: Nao Ling Decoction can obviously improve the expression of iNOS in hippocampal CA3 region of AD model and has a therapeutic effect on Alzheimer ’s disease rats.