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目的:观察参芎化瘀胶囊对脑缺血再灌注大鼠脑梗死体积、脑组织水含量及形态结构改变的影响,探讨参芎化瘀胶囊对大鼠急性脑缺血再灌注损伤的保护作用。方法:实验大鼠随机分为5组:假手术组、缺血再灌注组(模型组)、参芎化瘀胶囊高、中、低剂量治疗组(480,240,120 mg.kg-1)。ig给药7 d,每天1次,第7天ig 2 h后线栓法制作大脑中动脉阻断(MCAO)再灌注模型。采用TTC染色法观测脑梗死体积,干湿重法测量脑组织水含量,光镜和电镜观察脑形态结构改变情况。结果:与假手术组比较,模型组大鼠脑梗死体积增大,脑组织含水量升高,差异有显著性(均P<0.05);与模型组比较,参芎化瘀胶囊治疗组大鼠脑梗死体积、脑组织含水量有不同程度减少,差异有显著性(P<0.05,或P<0.01),其中以高剂量组效果最为显著。光镜和电镜下,参芎化瘀胶囊治疗组大鼠脑组织形态结构和超微结构损伤较模型组明显减轻,其中以高剂量组效果最为明显。结论:参芎化瘀胶囊对大鼠急性脑缺血再灌注损伤具有保护作用。
Objective: To observe the effect of Shenxionghuayu capsule on cerebral infarction volume, brain water content and morphological changes after cerebral ischemia-reperfusion in rats and to explore the protective effect of Shenxionghuayu capsule on acute cerebral ischemia-reperfusion injury in rats . Methods: The rats were randomly divided into 5 groups: sham operation group, ischemia reperfusion group (model group) and Shenxionghuayu capsule high, medium and low dose treatment group (480, 240, 120 mg.kg-1). ig administration for 7 days, once a day, on the seventh day after ig 2 h, the model of middle cerebral artery occlusion (MCAO) reperfusion was established. The volume of cerebral infarction was observed by TTC staining, the water content of brain tissue was measured by wet and dry method, and the morphological changes of brain were observed by light microscope and electron microscope. Results: Compared with the sham-operation group, the volume of cerebral infarction and the water content of brain tissue in model group were significantly increased (all P <0.05). Compared with model group, Shenxionghuayu capsule treatment group Cerebral infarction volume and brain water content decreased to some extent, the difference was significant (P <0.05, P <0.01), of which the effect of high dose group was the most obvious. Light and electron microscopy, Shenxionghuayu capsule treatment group rats brain morphology and ultrastructure damage significantly reduced compared with the model group, in which the effect of high-dose group is most obvious. Conclusion: Shenxionghuayu capsule has the protective effect on acute cerebral ischemia-reperfusion injury in rats.